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Gastric fluid pepsin assay is used to differentiate between neurogenic and gastric hypoacidity, the former sometimes having a normal pepsin level when there is too little or no gastric acid, and the latter having a deficiency of both hydrochloric acid and pepsin.
It is generally accepted that gastric hypoacidity is due to severe organic changes in the gastric mucosa, especially in pernicious anemia, achalasia, and absence of pepsin secretion, which are important diagnostic features. Pepsin secretion is often reduced in chronic gastritis, chronic gastric dilatation, and chronic duodecolitis.
Uses of Pepsin.
It is mainly used in the manufacture of fishmeal and hydrolysis of other proteins (e.g. soy protein), in the manufacture of cheese to characterize the curd (in combination with rennet), and in the prevention of cloudiness in the refrigeration of beer.
Pepsin is a digestive protease, secreted by gastricchiefcells in the stomach, which breaks down food proteins into small peptide fragments. The precursor of pepsin is known as pepsinogen. An acidic protease.
A protease derived from the gastric mucosa of animals. Capable of breaking down peptide bonds in proteins formed by aromatic or acidic amino acids, so it can catalyze the hydrolysis of casein, globulin, histone, keratin in animal horns, nails, and feathers, as well as catalyze the hydrolysis of plant proteins.
Pepsin is used as a digestive aid.
It is often used in symptoms such as indigestion caused by excessive consumption of proteinaceous food, reduced digestive function during the recovery period of illness, and pepsin deficiency due to chronic atrophic gastritis, gastric cancer, and pernicious anemia. However, it is forbidden to be used with alkaline drugs or aluminum sulfate.
It is used as a digestive aid for dyspepsia caused by pepsin deficiency or post-disease hypodigestion. In addition, it is used as a substitute for dry stomach film in the manufacture of lactic acid, and is also used in biochemical research and protein structure analysis.
Preparation of Pepsin.
Product Method of Pepsin.
The product was refined from porcine gastric mucosa, extracted with phosphate buffer, chromatographed on a DEAE-cellulose column, acidified and chromatographed again on Sephadex C-25 and Sephadex G-25 columns.
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