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Pectinase Powder Usage and Synthesis.Pectinase plays a very important role in juice production. In general, fruit juice after crushing contains pectin, cellulose and other solids, according to the size of the molecule, pectin plays the role of plant fibres, it will prevent or even make the liquid flow stop, so that the solid particles to remain suspended, the juice is in a uniform turbid state.
Therefore, the production of fruit juice must be pectin decomposition, so that its inactivity, in order to make the juice to achieve the purpose of clarification, and pectinase can play this role.
Pectinase is able to break down pectin, disintegrate plant cell walls and intercellular layers, making it easier to extract juice, and pectin is broken down into soluble galacturonic acid, which will also make cloudy juice become clear Chemicalbook clear. Pectinase is mainly found in higher plants and microorganisms, and is also found in some protozoa and insects. The main source of commercially available food-grade pectinase is the metabolites of Aspergillus niger.
Uses of Pectinase.
Enzyme preparation. Mainly used for juice clarification, improve the juice filtration speed, improve the juice yield, reduce the viscosity of juice, prevent the gelatinisation of puree and concentrated juice, strengthen the colour of grape juice and the comprehensive utilization of fruit and vegetable scraps. The highest reference dosage of 200mg/kg. such as grape juice with 0.2% pectinase at 40 ~ 42 ℃ for 3h, can be completely clarified. Grape pulp with 0.05% pectinase at 30 ~ 35 ℃ treatment, can increase the yield of 15%, improve the filtration speed of l times.
Pectinase catalyses the hydrolysis of methyl esters in pectin, as well as the breakdown of polygalacturonic acid into smaller polymers. It can be used as clarifying agent for beverages and also used for orange decapsulation. It can be used in canned oranges in sugar water (decapsulation), fruit wine, fruit juice, according to the production needs of the appropriate amount of use.
Pharmacological Action of Pectinase.
Product Method of Bulk Pectinase Powder.
This enzyme mainly comes from mould, and the industrial production mainly adopts Aspergillus niger as the production bacteria. It is cultivated in solid medium containing soybean meal, sucrose, apple pomace, etc.; then it is extracted with water, precipitated by organic solvent, then separated, dried and crushed to obtain the product. Commercial preparations also need to add diatomaceous earth, glucose and other fillers and preservatives, stabilisers and so on. It can also be obtained by extracting the mould after deep cultivation.
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